Title
luxS mutant regulation: quorum sensing impairment or methylation disorder?
Abstract
AI-2-mediated quorum sensing has been identified in various bacteria, including both Gram-negative and Gram-positive species, and numerous phenotypes have been reported to be regulated by this mechanism, using the luxS-mutant strain. But the AI-2 production process confused this regulatory function; some considered this regulation as the result of a metabolic change, which refers to an important metabolic cycle named activated methyl cycle (AMC), caused by luxS-mutant simultaneously with the defect of AI-2. Herein we hypothesized that the quorum sensing system-not the metabolic aspect-is responsible for such a regulatory function. In this study, we constructed plasmids infused with sahH and induced protein expression in the luxS-mutant strain to make the quorum-sensing system and metabolic system independent. The biofilm-related genes were investigated by real-time polymerase chain reaction (PCR), and the results demonstrated that the quorum-sensing completed strain restored the gene expression of the defective strain, but the metabolically completed one did not. This evidence supported our hypothesis that the autoinducer-2-mediated, quorum-sensing system, not the AMC, was responsible for luxS mutant regulation.
Year
DOI
Venue
2012
10.3390/s120506176
SENSORS
Keywords
Field
DocType
quorum sensing,AI-2,luxS,sahH
Quorum sensing,Gene,Biology,Gene expression,DNA methylation,Methylation,Plasmid,Mutant,Genetics,Mutation
Journal
Volume
Issue
ISSN
12
5.0
1424-8220
Citations 
PageRank 
References 
4
0.78
0
Authors
9
Name
Order
Citations
PageRank
Qian Wang140.78
Zhiyan He240.78
Yuejian Hu340.78
Yuntao Jiang440.78
Rui Ma540.78
Zisheng Tang640.78
Jingping Liang740.78
Zheng Liu840.78
Zhengwei Huang940.78